Re: "Why do my airpod pros disconnect from my Roku app every night at the exact same time?"
Guessing you would have checked that the AirPods are still well charged ... ?
You may want to check for interference from other wi-fi devices:
See: Resolve Wi-Fi and Bluetooth issues caused by wireless interference - Apple Support
If that hasn't helped:
Try turning off (better yet, use installers from their developers so they don't run continually in the background) any third-party (non-Apple) software, like unnecessary add-blockers, virus-checkers, cleaners, or the like, even VPN (if not supplied by an employer) as they often cause interference.
More info: If your device has network connectivity issues, check for VPN and other third-party security software - Apple Support
Third party apps that you find necessary need to be kept up-to-date to avoid compatibility issues
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Resource:
AirPods User Guide - Apple Support
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More support . info. if needed:
AirPods Support Website:
Find all the topics, resources, and contact options you need for your AirPods...
AirPods Support - Official Apple Support