Can I play Civilization V on a MacBook with Golden Gate hardware?

Yes, you can play Civilization V on a MacBook running macOS 27 Golden Gate.

You don't state your MacBook model (Pro, Air, Neo, M#) but because macOS Golden Gate drops support for Intel CPUs entirely, your Mac is natively powered by Apple Silicon (M-series or A18 Pro chips). While the official Mac version of Sid Meier's Civilization V on Steam is an Intel (x86-64) build, macOS Golden Gate still includes Rosetta 2 translation functionality.

How to Play It on macOS Golden Gate

• Install via Steam: Download and install Civilization V directly from Steam.
• Enable Rosetta 2: The first time you launch the game, macOS Golden Gate will prompt you to install Rosetta 2 if you haven't already. Once allowed, Rosetta will translate the Intel application code invisibly in the background

Note that macOS 27 Golden Gate is the last version of macOS that will run Civilization V. macOS 28 (coming Fall 2027) will drop support for that and other Intel apps entirely. I believe that Civilization VI will run natively on Apple Silicon.

A warning is triggered when you launch an Intel-only app, and you will see it every time you launch such an app. Launching a recently used Intel app again won't trigger it, but if enough time has passed then you'll see the warning again for that app. For example, I last used the SynologyAssistant app to check on my NAS over a month ago, and launching it generated the banner message.

To avoid this issue you need to run only Universal or Apple Silicon apps. For apps that are Intel only, contact the developers and push them to release updated versions of their apps (but keep in mind that they have over a year before their apps stop working on macOS). 

To see which of your apps are Intel and thus need to be updated or they won't run on macOS 28 (Fall 2027), hold the Option key and click the Apple menu, then select System Information (the top item). Select Applications in the Software section of the sidebar, then click on the Kind column header to sort them. 

Some apps aren't readily identifiable by the name, but when you select that app you will get more info. For example, 'commandFilter' is a component of the Epson printer drivers. Apps like that can be safely deleted. 

Here's what Apple has to say about updating your apps:

Learn how to update Intel-based apps for Apple silicon - Apple Support